Composite

Part:BBa_K3817017:Design

Designed by: Sun Ji   Group: iGEM21_UCAS-China   (2021-10-01)


SAM2-CBS inserted by Bleomycin pathway


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1361
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1361
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1361
    Illegal BglII site found at 1375
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1361
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1361
    Illegal NgoMIV site found at 2047
    Illegal NgoMIV site found at 2108
    Illegal AgeI site found at 751
    Illegal AgeI site found at 1408
    Illegal AgeI site found at 2655
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 495
    Illegal BsaI.rc site found at 1032


Design Notes

The regulation and selection of this pathway can be performed by bleomycin, and we can select colonies with resistance and thus select strains that can produce SAM efficiently.


Source

It comes from the Pichia pastoris GS115 and Saccharomyces cerevisiae.

References